Ubiquitinconjugating Enzyme H5c (UbcH5c), also known as Ubiquitinconjugating Enzyme E2D3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitinconjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid
sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins. For instance, UbcH5c/UBE2D3 is involved in the polyubiquitination and proteasomemediated degradation of the Nuclear Factor kappaB (NFkappaB) inhibitor, IkappaBalpha, and is implicated in NFkappaBdependent inflammation. UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication, and DNA damage responses.
Stock: X mg/ml (X μM) in PBS pH 7.3, 1 mM DTT
MW:
17 kDa (17 kDa UBE2D1)
Purity:
> 95% by SDS-PAGE under non-reducing conditions and visualized by Colloidal Coomassie Blue stain
USE：Recombinant Human UbcH5c/UBE2D3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5c/UBE2D3 concentration of 0.1-1 μM.
Storage: Store at -80°C. Avoid multiple freeze-thaw cycles.
