| Name | Ubiquitin Rabbit Polyclonal Antibody |
| Category | Polyclonal antibody(pAb); Primary antibody |
| Isotype | IgG |
| Purification | Peptide affinity purified |
| Immunogen |
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to Ubiquitin. |
| Gene ID | 7314 |
| SwissProt | P62988 |
| Synonyms |
40S ribosomal protein S27a; CEP80; Ribosomal protein S27a; RPS27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP80; UBIQ; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80; ubiquitin-CEP80 |
| Category | Ubiquitin and Ubiquitin-like proteins |
| Background |
Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway.Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via ifferent Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the biquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA epair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle egulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; ys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, NA-damage responses as well as in signaling processes leading to activation of the transcription factor NFkappa-B. |
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抗體
Ubiquitin Rabbit Polyclonal Antibody
| 產(chǎn)品編號 | 規(guī)格 | 價(jià)格 | 貨期 | |
| UBA-100 | 50 ul | ￥680 | 現(xiàn)貨 |
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| 來源 | 用途 | 交叉反應(yīng)性 | 分子量 |
| Rabbit | WB, ICC, IHC, IF | H, M, R | 10KDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry;
FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig;
Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
建議抗體使用時的稀釋比例如下(實(shí)際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
| WB | IF | IHC | ICC |
| 1：500-1：1000 | 1：200 | 1：200 | 1：200 |
保存條件：
Ubiquitin Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀釋液-20ºC或4ºC保存，一年有效。Western一抗稀釋液優(yōu)先推
薦4ºC保存，長期不使用可以考慮-20ºC保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng)：
? 如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請注意回收使用過的稀釋抗體?；厥盏目?br>體通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4ºC保存。
? 回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離
心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
? 提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得最佳
的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
? 本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
? 為了您的安全和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。
Western blot analysis of Ubiquitin on different cell lysates
using anti-Ubiquitin antibody at 1/1000 dilution.
Positive control: Lane 1: 293T Lane 2: HepG2 Lane 3: Hela
Lane 4: MCF-2.
