Formulation: 50 mM HEPES pH 8.0, 50mM NaCl
Purity: >95% as determined by SDS‐PAGE
Storage: Store at ‐80 ℃, avoid multiple freeze/thaw cycles
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- QQ:5161153
- 13844081467
- English
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蛋白質(zhì)與分子生物學(xué)
ubiquitin activating Enzyme (UBE1)
| 產(chǎn)品編號(hào) | 規(guī)格 | 價(jià)格 | 貨期 | |
| UBE-024 | 25ug | ￥1280 | 現(xiàn)貨 |
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Description
This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical compo-nent for the initiation of any in vitro conjugation reactions. Protein has tag at the N-terminus.
使用我公司的UBE1能夠?qū)⒎核卮呋纬煞核劓湥ㄈ缦聢D）。
